Antigen: Canine heartworm.

Monoclonal Designation: HW2-81.

Ig Isotype/subclass plus light chain: IgG1 kappa.

Specific reactivity: ether extracted parasite antigen, equal activity to male and female parasites. May recognize the heartworm larval antigen.

Properties:

Western blot: in reducing gels this MAb gives bands at 170 (fat), 155 (narrow); 140; bands at 98; 80; 70, and 62 kD, and a strong band at 33 kD.

ELISA: Excellent capture or probe with HW1-39 or HW2-83 MAbs.

Neutralization: Not known.

Affinity Chromatography: Not known.

Immunofluoresence: Positive.

Immunohistocytochemistry: Not known.

FACS Analysis: Not known.

Preparation and supply: SpA purified from tissue culture supernatant and supplied in PBS pH 7.2 with 0.05% sodium azide as preservative. Biotin conjugated MAb also usually available. Pricing: $(US)425.00 per mg SpA purified; $(US)495.00 per mg SpA purified and biotin conjugated. Minimum order 1.0 mg.

MAb HW2-81 References: None.

Other related heartworm MAbs available: HW1-39 HW2-83

N.B. CMI supplies anti-heartworm MAbs for research purposes only.Use of such MAbs for commercial diagnostic kit development is covered by US patent #4839275 (expires early 2003).